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石榴种质资源遗传多样性及亲缘关系的ISSR分析

赵丽华 李名扬 王先磊 刘华敏

果树学报2011,Vol.28Issue(1):66-71,6.
果树学报2011,Vol.28Issue(1):66-71,6.

石榴种质资源遗传多样性及亲缘关系的ISSR分析

Genetic diversity and genetic relationship of pomegranate (Punica granaturn) germplasm evaluated by ISSR markers

赵丽华 1李名扬 2王先磊 2刘华敏3

作者信息

  • 1. 西昌学院农业科学学院,四川西昌,615013
  • 2. 西南大学园艺园林学院,重庆市花卉工程技术研究中心,重庆,400715
  • 3. 重庆市园林绿化科学研究所,重庆,400715
  • 折叠

摘要

Abstract

ISSR markers was used to analyse the genetic relationship of 47 pomegranate cultivars .6 polymorphic primers were used for PCR, and amplied 120 bands in which 109 bands is polymorphic. The percent of polymorphic is 90.83%. The effective number of alleles (Ne), gene diversity of Nei (H), information index of Shannon (Ⅰ) were 1.294 5±0.309 4,0.189 7±0.161 8,0.309 1±0.219 8 respectively. Range of genetic distance (Dg) is from 0.075 0 to 0.400 0. The results showed higher level of genetic diversity among of pomegranate cultivars .Based on UPGMA, the 47 pomegranate cultivars were divided into 5groups. 15 special bands were detected, which may be used as reference of 11 pomegranate cultivars identity.

关键词

石榴/ISSR/种质资源/遗传多样性/亲缘关系

Key words

Pomegranates/ ISSR/ Germplasm/ Genetic diversity/ Genetic relationship

分类

农业科技

引用本文复制引用

赵丽华,李名扬,王先磊,刘华敏..石榴种质资源遗传多样性及亲缘关系的ISSR分析[J].果树学报,2011,28(1):66-71,6.

基金项目

四川省教育厅科研基金(08zc014) (08zc014)

西昌学院课题(YJSA0615) (YJSA0615)

果树学报

OA北大核心CSCDCSTPCD

1009-9980

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