果树学报2011，Vol.28Issue(1)：156-160,5.
中国野生华东葡萄抗白粉病相关基因(VpUSP)克隆及表达分析
Cloning and expression analysis of Vp USP gene in Chinese wild Vitis pseudoreticulata
摘要
Abstract
A USP(Universal stress protein) related EST sequence from the cDNA library constructed from powdery mildew-infected leaves of Baihe-35-1 was screened. Full length of this gene was obtained by RACE and was designated as VpUSP (GenBank accession number GU946975). It is 836 bp, which encodes 175 amino acids. Semi-quantitative RT-PCR was employed to study the expression of the gene. The results showed that constitutive expression of the gene was observed in susceptible lines. However, the gene could be induced by grapevine powdery mildew in resistant lines, and reached the climax after 48 h.It suggested that Vp USP is involved in the resistance to powdery mildew.关键词
中国野生葡萄/RACE(Rapid-ampllrfication of cDNA ends)/葡萄白粉病Key words
Vitis pseudoreticulata/ Rapid-amplrfication of cDNA ends (RACE)/ Powdery mildew分类
农业科技引用本文复制引用
赵莘,朱自果,徐炎,张朝红,王跃进..中国野生华东葡萄抗白粉病相关基因(VpUSP)克隆及表达分析[J].果树学报,2011,28(1):156-160,5.基金项目
国家自然科学基金((30971972) （(30971972）
现代农业产业技术体系专项(Nyeytx-30-ZP-06)公益性行业(农业)科研专项经费子课(200903044) （Nyeytx-30-ZP-06）
