中国当代儿科杂志2011，Vol.13Issue(1)：40-43,4.
氧化苦参碱对哮喘小鼠肺及脾脏组织中树突状细胞分布变化影响的研究
Effect of oxymatrine on the distribution of dendritic cells in lung and spleen tissues of asthmatic mice
摘要
Abstract
Objective To study the effect of oxymatrine (Oxy) on airway inflammation and the distribution of dendritic cells (DC) in lung and spleen tissues of asthmatic mice. Methods Fifty BALB/c mice were assigned into five groups (n = 10): an asthma model group, a dexamethasone (Dex) treatment group and three Oxy treatment groups (Oxy dose: 20, 40 and 80 mg/kg respectively). The histological changes of lung tissues were observed by hematoxylin and eosin staining. The expression of 33D1 antigen (a marker of DC ) in lung and spleen tissues were detected by immunohistochemical staining. Results The inflammatory reactions of the lung tissues in the Dex or Oxy treatment groups were less severe than those in the asthma model group. 33D1 antigen was remarkably expressed in the lung and spleen tissues of the asthma model group. After Dex treatment, the expression of 33D1 antigen in the lung and spleen tissues decreased significantly (P ＜ 0. 0 1). 33D1 antigen expression in the lung tissues was significantly reduced in all of the three Oxy treatment groups in a dose-dependent manner compared with that in the asthma model group ( P ＜ 0. 01 ). The treatment with Oxy of 40 and 80 mg/kg decreased significantly the 33D1 antigen expression in the spleen tissues ( P ＜ 0. 01 ). Conclusions Oxy can alleviate airway inflammation and reduce the number of DC in lung and spleen tissues of asthma mice, which may be contributed to the mechanism of Oxy for treatment of asthma.关键词
氧化苦参碱/气道炎症/脾脏/树突状细胞/小鼠分类
医药卫生引用本文复制引用
侯伟,刘海燕,杨旭东,周戬平,李丹,杨智毅,葛利吉..氧化苦参碱对哮喘小鼠肺及脾脏组织中树突状细胞分布变化影响的研究[J].中国当代儿科杂志,2011,13(1):40-43,4.基金项目
陕西省科技计划中药现代化项目(2008K-06) （2008K-06）
陕西省中医药管理局资助项目(No.03147). （No.03147）
