北京中医药大学学报2011，Vol.34Issue(1)：42-47,6.
脑脉通对老龄大鼠脑缺血/再灌注微血管生成及Flk-1表达的影响
Influences of Naomaitong on angiogenesis and Flk-1 expression in aged rats after cerebral ischemia-reperfusion *
摘要
Abstract
Objective To reveal the promoting mechanism of Naomaitong on cerebral angiogenesis after cerebral ischemia/reperfusion (I/R) in aged rats based on the expressions of fetal liver kianse-1 (Flk-1). Methods Male and aged SD rats were randomly divided into the sham-operation group, model group, nimodipine group and Naomaitong group. The microvessel density (MVD) of brain tissue, area of vascular field, and expressions of Flk-1 and Flk-1-mRNA were detected by using immunohistochemistry and hybridization in situ. Results Compared with the model group at the same time point, both MVD [ ischemia (I) for 3 h, and I/R for 1 d, 3 d and 12 d] and area of vascular field ( I for 3 h, and I/R for 3 d, 6 d and 12 d) in the Naomaitong group were increased (P ＜0. 05, P ＜0. 01). Compared with the nimodipine group, both MVD (I/R for 12 d) and the area of vascular field (I for 3h) in Naomaitong group were increased (P ＜0. 01 ). In the model group MVD lasted to descend from I for 3 h to I/R for 12 d, the are of vascular field reached peak after I/R for 1 d, then decreased quickly, and reached the lowest level after I/R for 12 d. The area of vascular field (I/R for 1 d) reached peak, then decreased quickly and to the lowest level after 12 days. In the Naomaitong group MVD increased significantly after I for 3 h, reached peak after I/R for 1 d, then started to decrease after, reached the lowest level after I/R for 6 d, and increased significantly after I/R for 12 d, and the area of vascular field increased significantly after I for 3 h, decreased to the lowest level after I/R for 1 d, increased a little after L/R for 3 d, decreased again after I/R for 6 d, and then increased again and recovered to the level of I/R for 3 d after I/R for 12 d. The expressions of Flk-1 in the Naomaitong group at all time points increased compared with the model group (P ＜ 0. 05, P ＜ 0. 01 ). Compared with the nimodipine group, expressions of Flk-1 in the Naomaitong group after I/R for 1 d, 6 d and 12 d increased (P ＜0. 05, P ＜ 0. 01 ), while decreased after I/R for 3 d (P ＜0. 01 ). In the model group the expression of Flk-1 began to increase after I for 3 h, reached peak after I/R for 1 d, and decreased rapidly after I/R from 1 d to 12 d. In the Naomaitong group the expression of Flk-1 performed better after I for 3 h, reached peak after L/R for 3 d, then decreased a little, but still kept a higher level after I/R for 12 d. Compared with the model group at the same time point, expression of Flk-1-mRNA increased ( P ＜ 0. 01 ) in the Naomaitong group, and increased after I/R for 6 d and 12 d ( P ＜0. 01 ) compared with the nimodipine group. In the model group the expression of Flk-1-mRNA reached peak after I/R for 1 d and decreased fast after I/R from I d to 12 d. In the Naomaitong group the expression of FIk-1-mRNA was higher after I for 3 h, reached peak after L/R for 1 d, then decreased gradually, and still kept a higher level after I/R for 12 d. Conclusion Naomaitong can promote angiogenesis after cerebral ischemia/ reperfusion in the aged, and the mechanism of which may be related to the increase of expressions of Flk-1 and Flk-1-mRNA.关键词
脑脉通/老龄/脑缺血/再灌注/血管生成/胚胎肝激酶-1/大鼠Key words
Naomaitong/ aged/ cerebral ischemia/reperfusion/ angiogenesis/ fetal liver kianse-1/ rats分类
医药卫生引用本文复制引用
刘轲,李建生,杨歆科,高剑峰,周友龙,赵跃武,刘政国,刘敬霞..脑脉通对老龄大鼠脑缺血/再灌注微血管生成及Flk-1表达的影响[J].北京中医药大学学报,2011,34(1):42-47,6.基金项目
国家自然科学基金项目(No.30371812),河南省杰出青年基金项目(No.0612000700) （No.30371812）
