重庆医学2011，Vol.40Issue(2)：115-117,3.DOI:10.3969/j.issn.1671-8348.2011.02.005
雷帕霉素对缺血再灌注大鼠肾小管上皮细胞凋亡的抑制作用
Effect of RPM on Bcl-2, Fas protein expression in renal ischemia-reperfusion injury of rats
郑传东 1苟欣 2胡兴平 1张力 1邓林1
作者信息
- 1. 四川省成都市第五人民医院泌尿外科,611130
- 2. 重庆医科大学附属第一医院泌尿外科,400016
- 折叠
摘要
Abstract
Objective To investigate the effect of RPM on Bcl-2 ,Fas protein expression at different ischemia-reperfusion points.Methods Acute ischemic renal injury model was established(ectomize right kidney,45min of left renal ischemia and reperfusion for 0,24,48,72 h). Fifty four normal male Wistar rats were randomly divided into 3 groups:sham-operated group (sham, without renal ischemia) ,ischemia-reperfusion group ( IR, withou t RPM), RPM and ischemia- reperfusion group ( RPM+ IR). Pathomorphological changes of renal ischemia-reperfusion injury were observed by HE stain,renal uhrastructure were measured and SABC immunohistochemical methods were used to detect the changes of expression of Bel-2,Fas. Results Bcl-2 and Fas concentration were significantly higher in IR group than those in sham group. As compared with IR group,Bcl-2 expression increased and Fas expression decreased significantly in RPM+IR group. In IR group local necrosis was found by HE stain. while similar changes were scarcely visible in RPM+IR group. The renal uhrastructure was greatly injured in IR group,meanwhile,it was obviously ameliorated in RPM+IR group. Conclusion RPM has significant effect on the up-regulation of Bcl-2 and down-regulation of Fas expression in renal ischemia-reperfusion injury.关键词
再灌注损伤/西罗莫司/细胞凋亡/Fas/Bcl-2/超微结构Key words
reperfusion injury/ sirolimus / apoptosis / Fas/ Bcl-2/ uhrastructure引用本文复制引用
郑传东,苟欣,胡兴平,张力,邓林..雷帕霉素对缺血再灌注大鼠肾小管上皮细胞凋亡的抑制作用[J].重庆医学,2011,40(2):115-117,3.
