大连工业大学学报2011，Vol.30Issue(1)：34-38,5.
生物表面活性剂产生菌的筛选及特性研究
Screening and characteristics of biosurfactant-producing bacteria
王健 1孙玉梅 1王培忠 1曹方1
作者信息
- 1. 大连工业大学,生物工程学院,辽宁,大连,116034
- 折叠
摘要
Abstract
Three bacteria were isolated from the soil contaminated by petroleum hydrocartbon, in which strain 31# was indentified as Pseudomonas, and strain 37# and strain 47# as Bacillus according to physiological and biochemical characteristics. The strains were cultured with paraffin as the sole carbon source, and the biomass, pH and surface tension of culture broth and cell hydrophobicity were determined. After fermentation of strains 31# , 37# and 47# , the surface tension of culture broth could reduce to 49.47, 58.00 and 56.04 mN/m, pH changed between 7.0 and 7.5. The strains displayed second growth and could reach their hydrophobicity maximum 3.09％, 1.49％ and 1.66％ respectively. The biosurfactant produced by strains 31 # , 37# and 47# was identified as rhamnolipid by thin-layer chromatography.关键词
生物表面活性剂产生菌/采油微生物/筛选/表面张力Key words
biosurfactant producer/ microbe for oil recovery/ screen/ surface tension分类
生物科学引用本文复制引用
王健,孙玉梅,王培忠,曹方..生物表面活性剂产生菌的筛选及特性研究[J].大连工业大学学报,2011,30(1):34-38,5.
