大连工业大学学报2011，Vol.30Issue(1)：13-17,5.
G8r菌产人参皂苷糖苷酶的酶性质及酶水解作用
Characterization of ginsenoside-glyosidase from Absidia sp. G8r
摘要
Abstract
The ginsenoside-glyosidase produced by Absidia sp. G8r was purified and characterized. The result indicated that its molecular weight is about 72 ku and the optimal temperature and pH for enzyme reaction were 30 and 5.0 respectively Enzyme is stable at 20-60 ℃, pH 3.0-7.0. Ions K+, Na+ , Ca2+ , Mg2+ , Zn2+ had no effect on the enzyme activity, while Cu2+ inhibited the enzyme activity. The enzyme hydrolyze Rb1 to C-K, in which Rb1 was hydrolyzed to Rd first in about 8 h, then Rd was hydrolyzed to F2 in about 18 h, and after that F2 was hydrolyzed to C-K. The Km of the enzyme are 5. 488, 5. 176, 5. 495 mmol/L, the Vmax of the enzyme are 1.524, 0. 904, 0. 447 mmol/(L · h).关键词
人参皂苷/人参皂苷糖苷酶/酶性质/水解作用Key words
ginsenoside/ ginsenoside-glyosidase/ enzymatic characteristics/ hydrolysis分类
轻工纺织引用本文复制引用
陈娇娇,孙键,金凤燮,鱼红闪..G8r菌产人参皂苷糖苷酶的酶性质及酶水解作用[J].大连工业大学学报,2011,30(1):13-17,5.基金项目
国家自然科学基金资助项目(30470055) （30470055）
辽宁省科学技术基金资助项目(20042132) （20042132）
辽宁省教育厅创新团队项目(2009T007). （2009T007）
