大连工业大学学报2011，Vol.30Issue(1)：5-9,5.
响应面优化产碱性蛋白酶菌株的产酶条件
Response surface optimization for production of alkaline protease of the fermentation conditions
顾艳丽 1张慧 1刘赛男 1李宪臻1
作者信息
- 1. 大连工业大学生物工程学院,辽宁大连,116034
- 折叠
摘要
Abstract
Response surface analysis was used to optimize the production of alkaline protease by strain I13 of the fermentation conditions with software Minitabl5. Plackett-Burman was adopted to screen out the important factors affecting alkaline protease production, which were inoculum size, yeast extract and temperature. This was utilized to approach the optimal region of the chitosanase production. The optimal composition of the medium was obtained by central composite design and response surface analysis: yeast extract of 10.2 g/L, temperature of 14.68 C, and inoculum size of 5.32％. The yield of alkaline protease production is 321.34 U/mL at the optical condition, more than 151％ before optimization.关键词
碱性蛋白酶/响应面分析/产酶条件Key words
alkaline protease/ response surface methodology/ fermentation conditions分类
轻工纺织引用本文复制引用
顾艳丽,张慧,刘赛男,李宪臻..响应面优化产碱性蛋白酶菌株的产酶条件[J].大连工业大学学报,2011,30(1):5-9,5.
