大连工业大学学报2011，Vol.30Issue(1)：30-33,4.
磁性壳聚糖微球固定化脂肪酶的研究
Immobilization of lipase onto magnetic chitosan microspheres
摘要
Abstract
The immobilized condition of lipase onto magnetic chitosan microspheres by cross-linking with glutaldehyde was optimized and the immobilized lipase was characterized. The optimum immobilizing time was 7 h, the optimum pH was 8, the optimum temperature was 50 ℃, and the optimum lipase amount were added 10 mg in 100 mg microspheres. Compared with free lipase, the thermal and acid-basic stabilities of immobilized lipase were increased appreciably. After 5 recycle of hydrolyzation, the activity of immobilized lipase remained 82.6％ of its initial activity. Magnetic chitosan microsphere was a kind of good support for immobilizing lipase.关键词
磁性微球/固定化酶/脂肪酶Key words
magnetic microspheres/ immobilized enzyme/ lipase分类
轻工纺织引用本文复制引用
常凯,王红英,孙井辉,钱斯日古楞..磁性壳聚糖微球固定化脂肪酶的研究[J].大连工业大学学报,2011,30(1):30-33,4.基金项目
辽宁省教育厅A类项目(2008063). （2008063）
