郑州大学学报（医学版）2011，Vol.46Issue(1)：7-9,3.
shRNA对EC9706细胞 p70S6K表达的影响
Effects of shRNA on expression of p70S6K in EC9706 cells
摘要
Abstract
Aim : To observe the effects of single-strand ( shRNAg) on the expression of p70S6K in EC9706 cells. Methods; According to the sequence of p70S6K mRNA,shRNAs were synthesized. Sense and antisense shRNA were annealed. The annealed products and the linear pSIREN-RetroQ-DsRed-Express were ligated by T4 ligase to create a recombi-nant plasmid pshRNA-p70S6K, and then the plasmid was transfected to EC9706 cells by Lipofectamine?2000. The expression levels of mRNA and p-p70S6K were analyzed, respectively, using RT-PCR and Western blot. EC9706 cells without tansfection was used as control. Results: The expression levels of p70S6K mRNA and p-p70S6JC in the 6 groups had significant differences (F = 106. 343,721. 632,P <0. 001). The expression of p70S6K mRNA decreased at 24,48 and 72 h after transfection(P < 0.05 ) , which come to the lowest at 48 h. The expression of p-p70S6K decreased at 24,48,72 and 96 h after transfection(P <0.05 ) .which come to the lowest at 48 h. Conclusion:p70S6K specific shRNA can downregulate the expressions of p70S6K mRNA and p-p70S6K in EC9706 cells.关键词
食管肿瘤/EC9706/哺乳动物雷帕霉素靶蛋白/核糖体40s小亚基S6蛋白激酶/短发夹RNAKey words
esophageal neoplasm/ EC9706/ mammalian target of rapamycin/ p70S6 kinase/ short hairpin RNA分类
医药卫生引用本文复制引用
郭三星,侯桂琴,李杰,张艳,贝维娟,薛乐勋..shRNA对EC9706细胞 p70S6K表达的影响[J].郑州大学学报（医学版）,2011,46(1):7-9,3.基金项目
国家自然科学基金资助项目 30901778 （）
教育部"十·五"、"211工程"重点学科建设项目教重字[2002]第2号 （）
