郑州大学学报（医学版）2011，Vol.46Issue(1)：88-91,4.
Aβ1-42单克隆抗体对阿尔茨海默病大鼠认知能力及脑组织Aβ和ChAT表达的影响
Effects of Aβ1-42 monoclonal antibody on cognitional ability and expression of Aβ, ChAT in brain tissue of Alzheimer's disease rat
黄月 1任秀花 2张杰文 1索爱琴1
作者信息
- 1. 河南省人民医院神经内科,郑州,450003
- 2. 郑州大学基础医学院人体解剖学教研室,郑州,450001
- 折叠
摘要
Abstract
Aim:To explore the effects and mechanism of Aβ1-42 monoclonal antibody(mAb) on cognitional ability in Alzheimer's disease (AD) rats. Methods: A total of 30 SD rats were divided into 3 groups: AD model group (12 rats), treatment group( 12 rate) and normal saline group(6 rats). Rats in AD model and treatment groups were made AD model with injection of Aβ1-42 peptide. Rats in treatment group were injected Aβ1-42 mAb through right side ventricle at 14th day after induced injection. Cognitional memory ability was tested by Morris water maze test, and the expression of Aβ and ChAT in brain tissue were examined by RT-PCR and immunohistochemistry. Results: Cognitional ability of rats among the 3 groups had significant differences( F = 23. 117 ,P <0.001 ) ,which improved in treatment group compared with AD moldel group(P < 0.05). The contents of Aβ and ChAT protein in brain tissues among the 3 groups had siginificant differences (F = 338.900 and 83.490,P <0.001). The expression of Ap in treatment group were lower than that in AD model group, but the expression of ChAT was the opposite. Conclusion: Aβ1 -42 mAb has obvious curative effect on AD model rats,which could reduce the accumulation of Aβ and adjust the avtivity of ChAT.关键词
阿尔茨海默病/β-淀粉样蛋白/单克隆抗体/乙酰胆碱转移酶/大鼠Key words
Alzheimer's disease /β amyloid protein/ monoclonal antibody/choline acetyltransferase/rat分类
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黄月,任秀花,张杰文,索爱琴..Aβ1-42单克隆抗体对阿尔茨海默病大鼠认知能力及脑组织Aβ和ChAT表达的影响[J].郑州大学学报（医学版）,2011,46(1):88-91,4.
