河南农业大学学报2011，Vol.45Issue(1)：46-50,5.
蜡梅SSR反应体系建立及引物筛选
Establishment of Chimonanthus SSR reaction system and its primer selection
摘要
Abstract
A study is made using Chimonanthus praecox as the materials, through the selection of template DNA, dNTP, primer concentration, Taq enzyme and annealing temperature for the impact of SSR amplification, establishing appropriate molecular markers reaction system of Chimonanthus SSR, and screening suitable SSR primers of Chimonanthus. The result showed that the appropriate annealing temperature of Chimonanthus SSR amplification was 55 ℃. In 20 μL reaction system, Chimonanthus template DNA, dNTP, primer concentration was 5 mg·L-1 ,0.2 mmol·L-1 and 0.5 μmol·L-1 ,Taq enzyme and 10 × Taq enzyme buffer was 2 U and 2.5 μL. Using the reaction system, 41 pairs of SSR primers for Chimonanthus SSR amplified were screened from 417 pairs different woody plants primers.关键词
素心蜡梅/SSR/反应体系/引物筛选Key words
Chimonanthus praecox/ SSR/ reaction system/ primer selection分类
农业科技引用本文复制引用
赵明晓,范国强..蜡梅SSR反应体系建立及引物筛选[J].河南农业大学学报,2011,45(1):46-50,5.基金项目
林业公益性行业科研专项(201004002) （201004002）
