安徽农业科学2011，Vol.39Issue(2)：764-765,769,3.
高纯度银杏黄酮的制备
Preparation of High-Purity Flavonoids from Ginkgo biloba L.
摘要
Abstract
[ Objective ] To prepare highly purified flavonoids of Ginkgo biloba L. , so as to explore a large - scale preparation process line of high purity flavonoids from Ginkgo biloba L.. [ Method ] Purified with chromatographic column with packing of ODS, effects of mobile phase ratio, concentration temperature and drying temperature on yield of flavonoids from Ginkgo biloba L. were investigated. [ Result] The optimum preparation conditions were as follows: target fraction of methanol to 0.5％ phosphoric acid ratio of 5:5 and 7: 3, concentration temperature and drying temperature of 45 °C. The content of flavonoids from Ginkgo biloba L. was more than 90％, and the yield reached 78.3％ under the optimal preparation process. [ Conclusion ] High-purily flavonoids from Ginkgo biloba L. were obtained, which provided an effective method for the preparation of highly purified Ginkgo biloba L. flavonoids.关键词
银杏总黄酮/高纯度/梯度洗脱/浓缩温度/干燥温度/高效液相色谱法Key words
Ginkgo biloba L. Flavonoids/ High purity/ Gradient elution/ Concentration temperature/ Drying temperature/ HPLC分类
农业科技引用本文复制引用
张伟,李芊,林炳昌..高纯度银杏黄酮的制备[J].安徽农业科学,2011,39(2):764-765,769,3.基金项目
辽宁省教育厅团队项目(2008T093) （2008T093）
辽宁科技大学2010年度校青年基金团队项目子课题(20100602). （20100602）
