安徽农业科学2011，Vol.39Issue(2)：1049-1050,2.
不同培养体系对牛卵母细胞体外受精的影响
Effects of Different Culture Systems on in Vitro Fertilization of Oocyte in Bovine
摘要
Abstract
[ Objective ] To investigate the optimal culture system for in vitro fertilization (IVF) of oocyte in bovine. [ Method ] The IVF of mature bovine oocytes was conducted and the zygotes were cultured in vitro with different culture systems. The effects of different culture systems on fertilization rate and cleavage rate were observed. [ Result] In the transitional culture system, with TALP medium as fertilization medium,the fertilization rate and cleavage rate were increased to 77.8％ and 55.6％, respectively. [ Conclusion] The transitional culture system, with TALP medium as fertilization medium and with 60％ TALP medium + 40％ M-199 medium +5％ FCS as IVF zysote culture medium is the optimal culture system for IVF of oocyte in bovine.关键词
牛/卵巢/卵母细胞/体外受精/培养体系Key words
Bovine/ Ovary/ Oocyte/ In vitro fertilization/ Culture system分类
农业科技引用本文复制引用
刘新峰,郝志明,史远刚,赵明辉,王敏,李正锋..不同培养体系对牛卵母细胞体外受精的影响[J].安徽农业科学,2011,39(2):1049-1050,2.基金项目
天津农学院科学研究发展基金计划重点项目. （）
