华西口腔医学杂志2011，Vol.29Issue(1)：9-12,4.DOI:10.3969/j.issn.1000-1182.2011.01.003
大蒜素对尼古丁致人牙周膜成纤维细胞氧化毒性的保护作用
Protective effect of allicin on human periodontal ligament cells with nicotine-induced oxidative damage
摘要
Abstract
Objective To explore the protective effect of allicin on nicotine-induced oxidative damage to human periodontal ligament cells (HPDLCs). Methods l) Establish nicotine-induced oxidative damage model on HPDLCs. Use water-soluble tetrazolium (WST) colorimetric method to find out the nicotine concentration (X) that could inhibit HPDLCs' growth for the following experiments. 2)HPDLCs of the fifth passage were divided into 5 groups: The control group, the nicotine group and the nicotine+allicin groups(the concentration of allicin was 15, 30, and 60 μg·mL-1 respectively). Different kinds of culture media were added. Similarly, use WST colorimetfic method to choose the allicin concentration (Y) that could significantly improve the survival rate of HPDLCs. 3)HPDLCs were divided into 3 groups: The control group, the nicotine group, the nicotine+allicin group and different media were added. The glutathion(GSH) concentrations in HPDLCs were determined in 1, 4, 8, 12 and 24h respectively. Results 0.8mg·mL-1 nicotine could inhibit the HPDLCs survival rate significantly (77％ of the control, P＜0.05). But 60μg·mL-1 allicin could prevent the inhibition effects evidently, improving the survival rate to 112％ of that of the nicotine group(P＜ 0.05) and reaching the survival rate level of control group (P＞0.05). The GSH concentrations of nicotine+allicin group were higher than that of the nicotine group always (P＜0.05) and by 82％ at 8 h after culture, but had no difference with that of the control group(P＞0.05). Conclusion 60 μg·mL-1 allicin can protect the HPDLCs against oxidative damage induced by nicotine.关键词
人牙周膜成纤维细胞/尼古丁/氧化毒性/大蒜素/谷胱甘肽Key words
human periodontal ligament cells/ nicotine/ oxidative damage/ allicin/ glutathion分类
医药卫生引用本文复制引用
李斌龙,谢晓莉,彭解英,罗小良,靳路远..大蒜素对尼古丁致人牙周膜成纤维细胞氧化毒性的保护作用[J].华西口腔医学杂志,2011,29(1):9-12,4.基金项目
湖南省科技厅基金资助项目(2010FJ3067) （2010FJ3067）
中南大学理科发展基金资助项目(1773-206001081) （1773-206001081）
