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CdS量子点自组装膜对DNA的界面传感

柯培林 孙向英

华侨大学学报(自然科学版)2011,Vol.32Issue(1):48-52,5.
华侨大学学报(自然科学版)2011,Vol.32Issue(1):48-52,5.

CdS量子点自组装膜对DNA的界面传感

Prepariton of CdS Self-Assembled Monolayers and Interfacial Sensing for DNA

柯培林 1孙向英1

作者信息

  • 1. 华侨大学,材料科学与工程学院,福建,泉州,362021
  • 折叠

摘要

Abstract

CdS quantum dots (QDs) was synthesized directly in aqueous solution stabilized by thioglycolic acid. The CdS QDs was assemblied onto the surface of quartz substrate by the electrostatic interaction between the CdS QDs, γ-amin-opropyl-triethoxysilane and chitosan, and CdS QDs nanofilm was constructed with layer by layer assembly technique The obtained self-assemble multilayer film was characterized by fluorescence spectra and fluorescence microscope. Further, the self-assembled multilayer film could be used as a fluorescence sensor for DNA. Experimental investigation shows that the fluorescence intensity of the Quartz/APES/CdS SAMs decreased with the increase of the concentration of DNA, and displayed a good linear relationship within a certain concentration. In addition, the correlation coefficient was 0. 996 17, and the detection limit was as low as 38. 6 ng · L-1 , qualifying the monolayer very useful in detection of DNA.

关键词

CdS/量子点/自组装膜/界面荧光/DNA/界面传感

Key words

CdS/ quantum dots/ self-assembled multilayers films/ interfacial fluorescence/ DNA/ interfacial sensing

分类

化学化工

引用本文复制引用

柯培林,孙向英..CdS量子点自组装膜对DNA的界面传感[J].华侨大学学报(自然科学版),2011,32(1):48-52,5.

基金项目

国家自然科学基金资助项目(20955001) (20955001)

福建省自然科学基金资助项目(D0710017,D0810016) (D0710017,D0810016)

教育部留学回国人员科研启动项目(2008年度) (2008年度)

华侨大学学报(自然科学版)

OA北大核心CSTPCD

1000-5013

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