渔业科学进展2011，Vol.32Issue(1)：60-66,7.
pH胁迫对日本对虾非特异性免疫因子及RNA/DNA比值的影响
Effects of pH stress on non-specific immune factors and RNA/DNA ratio of Marsupenaeus japonicus
摘要
Abstract
The effects of pH stress on non-specific immune factors and the RNA/DNA ratio of Marsupenaeus japonicus were investigated. It was found that the activity of total nitric oxide synthase (TNOS) in M. japonicus of low pH group (pH 7. 2) and high pH group (pH 9.2) reached the maximum at 3h and 12h, respectively. The activity of inducible nitric oxide synthase (INOS) in pH stress groups reached the maximum at 3h, then gradually declined and stabilized at 72h. The activity changes of phenoloxidase (PO) in M. japonicus under both the low and high pH conditions showed that they reached the peak value at l2h, gradually decreased thereafter, and became stable after 72h. Activities of lysozyme, superoxide dismutase, and glutathione peroxidase in M. japonicus decreased gradually under pH stress. The changes of non-specific immune enzymatic activity showed that the immune adaptive activity in M. japonicus exposed to the high pH stress was lower than those exposed to the low pH stress. Moreover, the RNA/DNA ratio in the muscle of M. japonicus under the pH stress conditions was significantly lower than that of the control group, supposedly due to the effects of pH stress on metabolism of M. japonicus.关键词
pH胁迫/日本对虾/非特异性免疫因子/RNA/DNAKey words
pH stress/ Marsupenaeus japonicus/ Non-specific immune factors/RNA/DNA分类
农业科技引用本文复制引用
赵先银,李健,李吉涛,何玉英,张喆,常志强..pH胁迫对日本对虾非特异性免疫因子及RNA/DNA比值的影响[J].渔业科学进展,2011,32(1):60-66,7.基金项目
公益性农业行业科研专项(200803012)、国家虾产业技术体系(nycytx-46)和国家863计划项目(2010AA10A401)共同资助 （200803012）
