吉林农业大学学报2011，Vol.33Issue(1)：47-50,4.DOI:CNKI:22-1100/S.20101215.1412.0000
龙牙楤木SE基因克隆与植物表达载体构建
Cloning of Squalene Epoxidase Gene in Aralia elata and Construction of Its Plant Expression Vector
摘要
Abstract
The squalene epoxidase (SE) gene from Aralia elata (AeSE) was amplified by RT-PCR.The result showed that the full-length cDNA of AeSE gene (GenBank accession Number: GU354314) is 1 682 bp and contains a 1 644 bp open reading frame (ORF) encoding a polypeptide of 547 amino acids.The SE proteins from Aralia elata, Panax ginseng, Panax notoginseng and Gynostemma pentaphyllum showed more than 90％ identities based on amino acid sequences alignment. The plant expression vector pCAMBIA 3301 - AeSE was constructed.关键词
龙牙楤木/鲨烯环氧酶(SE)基因/植物表达载体/三萜皂苷Key words
Aralia elata/ squalene epoxidase gene/ plant expression vector/ triterpenoid saponin分类
生物科学引用本文复制引用
成慧,杨光,刘雅婧,赵春彦,原亚萍,吴颖..龙牙楤木SE基因克隆与植物表达载体构建[J].吉林农业大学学报,2011,33(1):47-50,4.基金项目
长春市科技计划项目(08YJ29) （08YJ29）
