吉林农业大学学报2011，Vol.33Issue(1)：69-73,5.
应用巢式PCR-RFLP方法鉴别我国常见的几种隐孢子虫
Distinction of Cryptosporidium parvum, C.andersoni, C.baileyi and C.suis by Nested PCR-RFLP
摘要
Abstract
In the present study, we have described a Nested PCIR - RFLP assay for the distinction of C.parvum, C.andersoni, C.baileyi and C.suis.The fragment.- of 212,213,213,213,210 bp targeting the 18S rRNA gene were respectively amplified by Nested PCR.When PCR products were digested with Taq Ⅰ ,BstU Ⅰ and Mse Ⅰ, C.parvum, C.andersoni, C.baileyi and C.suis can be effectively discriminated based on RFLP restriction maps.The study provided a novel assay for the taxonomy of Cryptosporidium and molecular epidemiology survey of Cryptosporidiosis.关键词
隐孢子虫/巢式PCR-RFLP/鉴别方法Key words
Cryptosporidium/ nested PCR - RFLP/ distinction method分类
农业科技引用本文复制引用
李巍,张西臣,李建华,李淑红,宫鹏涛,杨举..应用巢式PCR-RFLP方法鉴别我国常见的几种隐孢子虫[J].吉林农业大学学报,2011,33(1):69-73,5.基金项目
国家科技支撑计划项目(2007BAD40B05) （2007BAD40B05）
