湖南农业大学学报（自然科学版）2011，Vol.37Issue(1)：82-85,4.DOI:10.3724/SP.J.1238.2011.00082
诊断抗原的纯化在牛γ-干扰素ELISA法中的应用
Purification in antigen and application in diagnosis of IFN-γ-ELISA detection of bovine tuberculosis
摘要
Abstract
The test results of the bovine tuberculin skin test (TST), acid-fast stain test, ELISA test and bovine IFN-γELISA diagnosis test respectively were compared for detection of tuberculosis of them, three stimulators, detected by IFN-γ-ELISA including bovine PPD, avian PPD and bovine PPD at affinity chromatography were compared in exploration for application of purification of diagnostic antigen in the IFN-Υ-ELISA method.Using acid-fast staining as the standard, the sensitivity of IFN-Υ-ELISA detection reached 83.33％(5/6), and its specificity reached 98.14％(53/54), the consistency reached 98.33％ (58/60).As the other result, acid-fast stain detected a positive sera with high OD value and produced a positive result using bovine IFN-γ-ELISA in which the antigen was not purified at affinity chromatography, but the result was negative with low OD value using bovine IFN-γ-ELIS in which the antigen was purified at affinity chromatography.In conclusion, the miscarriage of justice caused by infected with Mycobacterium bovis and environmental Mycobacterium simultaneouly could be prevented using bovine IFN-γ-ELISA in which the antigen was purified at affinity chromatography.So this method has great potential to replace TST for development and application prospects in China.关键词
牛结核/皮内变态反应/抗酸染色/牛结核ELISA/γ-干扰素ELISA法/亲和纯化Key words
bovine tuberculosis/ tuberculin skin test (TST)/ acid-fast stain/ PPD-ELISA/ IFN-γ ELISA/ affinity chromatography分类
农业科技引用本文复制引用
邱美珍,周望平,肖兵南,杜丽飞,胡述光,刘毅,陈江..诊断抗原的纯化在牛γ-干扰素ELISA法中的应用[J].湖南农业大学学报（自然科学版）,2011,37(1):82-85,4.基金项目
国家科技支撑计划项目(2006BAD04A15-2) （2006BAD04A15-2）
