华西口腔医学杂志2011，Vol.29Issue(1)：66-70,5.DOI:10.3969/j.issn.1000-1182.2011.01.016
沉默DNA结合抑制蛋白-1基因对腺样囊性癌细胞生长和侵袭能力的影响
Effects of silencing inhibitor of DNA binding-1 gene on the growth and invasiveness of adenoid c stic earcinoma cells
摘要
Abstract
Objective To investigate the role of inhibitor of DNA binding-1 (Id-1) gene in adenoid cystic carcinoma cell growth and invasion behavior.Methods With salivary adenoid cystic carcinoma cell lines ACC-M and ACC-2, dedected Id-1 gene expression was screened with immunofluorescence assay.After Id-1 mRNA knockingdown using small interfering RNA, RT-PCR and Western blot were used to detect the different expressions before and after interference, and the growth of cells before and after interference was deceted using the MTT assay, and the cell invasion ability was checked with the use of Transwell chamber assay.Results Id-1 were both expressed in the ACC-M and ACC-2, and the expression in ACC-M was higher than that in ACC-2.After Id-1 RNA interference, the growth and invasiveness of ACC-M and ACC-2 were inhibited with the restrained degree in ACC-M much stronger than that in the ACC-2.Conclusion In view of the important role of Id-1 in the behavior of growth and invasion in ACC cell, interfering the expression of Id-1 gene is expected to be a novel and effective means for the treatment of adenoid cystic carcinoma.关键词
DNA结合抑制蛋白-1/腺样囊性癌/增殖/侵袭Key words
inhibitor of DNA binding-1/ adenoid cystic carcinoma/ proliferation/ invasion分类
医药卫生引用本文复制引用
刘培,张向红,胡振生,孙善珍,刘少华,魏奉才..沉默DNA结合抑制蛋白-1基因对腺样囊性癌细胞生长和侵袭能力的影响[J].华西口腔医学杂志,2011,29(1):66-70,5.基金项目
国家自然科学基金资助项目(30672339) （30672339）
教育部留学回国人员科研启动基金资助项目(2007) （2007）
