华西口腔医学杂志2011，Vol.29Issue(1)：13-16,4.DOI:10.3969/j.issn.1000-1182.2011.01.004
钛片表面粗糙度和氧化膜对成骨细胞黏附影响的体外研究
Effect of surface roughness and titanium dioxide layers on commercially pure titanium on attachment of osteoblasts
周屹立 1丁仲鹃 1唐玲2
作者信息
- 1. 昆明医学院口腔医学院,口腔修复学教研室,昆明,650031
- 2. 昆明市第一人民医院,口腔修复科,昆明,650032
- 折叠
摘要
Abstract
Objective To study the effects of surface roughness and titanium dioxide (TiO2) layers on commercially pure titanium(cp-Ti) substrates on attachment of osteoblasts in vitro. Methods 250 pure titanium slices were divided into five groups. Osteoblasts were cultured on five cp-Ti substrates of ground, which blasted with 108-130 μm (S1),216-301 μm(S2), 356-411 μm(S3) TiO2 particles and titanium-sprayed plasma(TPS) surfaces, surfaces prepared by hand grinding with SiC paper to 600 grits served as control (So). Surface average roughness and the TiO2 film structure was evaluated. For morphology and attachment measurement, osteoblasts were cultured for 1, 4, 12 and 24 h,evaluated by scanning electronic microscope (SEM) observation and MTT assay. Results Osteoblasts spread well on the titanium surfaces. Further more, osteoblasts spread more well on S3 surfaces. After 1 and 4 h culture, the number of cells on S3 surfaces was the highest (P＜0.05). The number of cells on S3 surfaces was the same (P＞0.05) as TPS surfaces and higher than other groups (P＜0.05) after 12 and 24 h. The number of cells of all experimental groups were higher than So surfaces after 4, 12 and 24 h (P＜0.05). Conclusion It was concluded that thecoarse TiO2 particles blasted surface would optimize initial osteoblast responses.关键词
成骨细胞/粗糙度/二氧化钛/黏附Key words
osteoblast/ roughness/ titanium dioxide layer/ attachment分类
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周屹立,丁仲鹃,唐玲..钛片表面粗糙度和氧化膜对成骨细胞黏附影响的体外研究[J].华西口腔医学杂志,2011,29(1):13-16,4.
