湖南农业大学学报（自然科学版）2011，Vol.37Issue(1)：60-64,5.DOI:10.3724/SP.J.1238.2011.00060
猪繁殖候选基因HoxA10的克隆及表达分析
Cloning and expression analysis of HoxA10,a candidate gene influencing reproduction traits in pigs
摘要
Abstract
As a key member of Hox gene family, the Homeobox A1O gene (HoxA1O) is an important candidate gene influencing reproduction traits in pigs, which plays important roles in embryonic development and cell differentiation. In this paper, HoxA1O gene was cloned from a Landrace pig by RT-PCR, and different tissues from the pig were tested by real-time PCR to determine the tissue-specific expression pattern of HoxA1O. Results showed that the cloned HoxA1OcDNA of pig was 538 bp long, and it contained an open reading frame (ORF) of 285 bp encoding a peptide of 94 amino acid residues which showed 98.9％ and 97.9％ sequence identity to that of human and mouse respectively. In all tested pig tissues, HoxA1O expressed predominantly in forward leg muscle, followed by kidney, uterus, back leg muscle, oviduct,large intestine and abdominal fat. And little or no Expression of HoxA1O was detected in hypothalamus, cerebrum,cerebellum, thalamus, ovary, lung, stomach, small intestine, dorsal muscles and back fat.关键词
猪/HoxA10基因/cDNA克隆/组织表达分类
农业科技引用本文复制引用
周晓宁,方梅霞,何小梅,聂庆华,张细权..猪繁殖候选基因HoxA10的克隆及表达分析[J].湖南农业大学学报（自然科学版）,2011,37(1):60-64,5.基金项目
农业部转基因生物新品种培育重大专项(2008ZX08006-005 （）
2009ZX08009-145B) （）
粤港关键领域重点突破项目(2008A02) （2008A02）
