家畜生态学报2011，Vol.32Issue(1)：1-4,4.
锌指核酸酶技术研究进展
Advancement of Zinc Finger Nuclease Technique
摘要
Abstract
Recombinant Zinc finger nuclease (ZFN), which is a fusion protein that contains domains of zinc finger protein and cleavage domain of FokI endonuclease, is designed to cut DNA at specific sequences and occur a double-strand break (DSB), the ZFN technique improves efficiency of gene targeting process of replacing and modifying gene based on homologous recombination, and provides a powerful platform for transgenic and gene therapy research. This review summarizes its research status,application, and prospects development of ZFN technique.关键词
锌指核酸酶/基因打靶/转基因/基因治疗Key words
Zinc finger nuclease/ gene targeting/ transgene/ gene therapy分类
农业科技引用本文复制引用
王晓静,武建明,王洪梅,李秋燕,何成强,刘晓,高运东,仲跻峰,何洪彬..锌指核酸酶技术研究进展[J].家畜生态学报,2011,32(1):1-4,4.基金项目
泰山学者海外特聘专家(何洪彬) （何洪彬）
国家转基因重大专项(2009ZX08007-006B) （2009ZX08007-006B）
国家自然科学基金(31072160),山东省农业重大应用技术创新课题(何洪彬) （31072160）
山东省科技攻关课题(2009GG20002032) （2009GG20002032）
山东省自然基金(Y2008D20) （Y2008D20）
