湖南农业大学学报（自然科学版）2011，Vol.37Issue(1)：26-30,5.DOI:10.3724/SP.J.1238.2011.00026
侧孢芽孢杆菌2-Q-9菌株外泌抗菌肽的纯化与鉴定
Purification and characterization of antimicrobial peptide secreted by Bacillus laterosporus 2-Q-9
摘要
Abstract
Bacillus laterosporus stranin 2-Q-9 secreted a high level of antimicrobial peptides when cells were grown in liquid NB media at 28 ℃ for 24 h. Purification was carried out by protein precipitation with 75％ ethanol, anion-exchange chromatography and cation-exchange chromatography sequentially. The relative molecular weight and isoletric point of antimicrobial peptide BL2Q9 were 7 800 and 10.2 respectively. The sequencing results of MS/MS showed that the antimicrobial peptide was a new antimicrobial peptide (AMP) with low amino acid sequence similarity among the known AMPs in database.关键词
侧孢芽孢杆菌/外泌抗菌肽/纯化与鉴定/质谱测序分类
生物科学引用本文复制引用
陈武,彭曙光,周清明,黎定军,罗宽..侧孢芽孢杆菌2-Q-9菌株外泌抗菌肽的纯化与鉴定[J].湖南农业大学学报（自然科学版）,2011,37(1):26-30,5.基金项目
湖南省自然科学基金项目(BK0431) （BK0431）
湖南省教育厅青年项(03B012) （03B012）
