渔业科学进展2011，Vol.32Issue(1)：104-108,5.
不同毒力溶藻弧菌脂多糖的多态性研究
The polymorphism of lipopolysaccharides isolated from Vibrio alginolyticus strains with different virulence
摘要
Abstract
Lipopolysaccharides (LPS) were extracted from ten Vibrio alginolyticus strains with different virulence. Five of them were virulent strains, named as HN08811,HN08155, HN08813, HN08335, and HN07006 and the others were non-virulent strains,named as HN08805, HN08307, HN08232, HN08201, and TG06003. The results of SDSPAGE (sodium dodecyl sulfate polyacrylamide polyacrylamide gel electrophoresis) with 10 μg LPS showed that LPS of V. alginolyticus strains with different virulence had abundant polymorphism. The polymorphism of lipid A section of each LPS was difficult to distinguish. However, for the O-antigen section, 7～8 clear bands of LPSs from virulent strains were observed except for HN07006. LPSs from all the non-virulent strains had discernable slender and intensive bands. This method not only creates a new molecular typing method for rapid identification of virulent strains of V. alginolyticus, but also provides scientific theoretical data for the analysis of serotype of V. alginolyticus.关键词
溶藻弧菌/脂多糖/毒力/多态性Key words
Vibrio alginolyticus/ Lipopolysaccharides (LPS)/ Virulence/ Polymorphism分类
生物科学引用本文复制引用
宣雄智,谢珍玉,周永灿,王世锋,吴学贵..不同毒力溶藻弧菌脂多糖的多态性研究[J].渔业科学进展,2011,32(1):104-108,5.基金项目
国家自然科学基金项目(30660144,30760190)、海南省高校硕士研究生创新科研课题(Hxwsy2008-01)和海南大学2009年度科研项目(hd09xm57)共同资助 （30660144,30760190）
