湖南农业大学学报（自然科学版）2011，Vol.37Issue(1)：86-89,4.DOI:10.3724/SP.J.1238.2011.00086
高速逆流色谱法分离前胡提取物中香豆素类成分
Separation of coumarins with function from extract of radix peucedani through HSCCC
摘要
Abstract
The BALB/c mice were immunized with DHV of allantoid liquid in duck embryo which were purified by freezing and melting repeatedly, dealt with by chloroform, concentrated by PEG and overspeed membrane filtrating.Eight hybridoma cell lines were obtained after detecting and 3 sub-cloning by I-ELISA. They were named DHV-1,DHV-6, DHV-7, DHV-8, DHV-9, DHV-IO, DHV-11 and DHV-I2 respectively. The ascites titres of these MAbs were between 1 : 2 000 and 1 : 512 000. The ascites had no cross reaction with other antigen and the normal allantoid liquid in duck embryo. MAbs belonged to IgG with K -chain, neutralization test and young duck pretection test indicated that neutralization characters of DHV-6, DHV-7, DHV-9, DHV-1O are stronger.关键词
白花前胡/高速逆流色谱/前胡香豆素Ⅱ/北美芹素/白花前胡甲素/补骨脂素分类
化学化工引用本文复制引用
蔡海林,杜良伟,刘祥英,陈桂华,王彦辉,柏连阳..高速逆流色谱法分离前胡提取物中香豆素类成分[J].湖南农业大学学报（自然科学版）,2011,37(1):86-89,4.基金项目
国家自然科学基金项目(30971951) （30971951）
湖南农业大学稳定人才基金项目(09WD18) （09WD18）
