基础医学与临床2011，Vol.31Issue(1)：68-72,5.
赖氨大黄酸通过激活JNK诱导宫颈癌HeLa细胞凋亡
Rhein lysinate induces apoptosis of human cervical cancer HeLa cells by activating c-Jun N-terminal kinase (JNK)
甄永占 1林雅军 2骆广玲 3赵毓芳 1高俊玲1
作者信息
- 1. 华北煤炭医学院,基础部,组织学与胚胎学教研室,河北,唐山,063000
- 2. 卫生部北京医院,卫生部北京老年医学研究所,北京,100730
- 3. 开滦医疗集团林西医院妇产科,河北,唐山,063000
- 折叠
摘要
Abstract
Objective To investigate the effect of rhein lysinate (RHL) on inducing human cervical cancer cell line HeLa apoptosis and the role of activation of c-Jun N-terminal kinase (JNK) in cell apoptosis.Methods MTT assay was used to detect HeLa cell proliferation.Cell apoptosis was analyzed by flow cytometry.The protein associated with apoptosis and the protein phosphorylation of JNK was detected by Western blot.Results RHL remarkably inhibited cervical cancer HeLa cells proliferation.The IC50 value for 48 and 72 h treatment was 81.18 μmol/L and 72.38 μmol/L respectively.Apoptosis of HeLa cells was induced by RHL in a dose dependent manner.RHL triggered HeLa cells apoptosis by increasing the level of cleaved poly ADP-ribose polymerase (PARP)and caspase-3/7.In addition, activation JNK was a critical mediator in RHL-induced growth inhibition.Inhibition of the phosphorylation of JNK by pharmacological inhibitors reversed the RHL-induced growth inhibition through decreasing the level of cleaved PARP and caspase-3/7.Conclusion RHL suppresses HeLa cells growth and induces apoptosis through activation of JNK-caspase-PARP signal pathway, with the potential to be developed as a chemotherapeutic agent for cervical cancer.关键词
赖氨大黄酸/细胞凋亡/宫颈癌/JNKKey words
rhein lysinate/ cervical cancer/ apoptosis/ JNK分类
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甄永占,林雅军,骆广玲,赵毓芳,高俊玲..赖氨大黄酸通过激活JNK诱导宫颈癌HeLa细胞凋亡[J].基础医学与临床,2011,31(1):68-72,5.
