基础医学与临床2011，Vol.31Issue(1)：32-36,5.
小剂量VCR联合Endostar抑制PLA-802细胞系VEGF表达
The effect of the combination of small dosage of VCR and Endostar on the expression of VEGF in PLA-802 cell line
摘要
Abstract
Objective To explore the effect of the combination of small dosage of VCR and Endostar(Endo) on rhabdomyosarcoma cells and its mechanisms. Methods PLA-802 was cultured and treated with small dosage of VCR, Endostar singly or both. MTT was used to examine the cells proliferation, and flow cytometry was used for the checking cell cycle. While, RT-PCR and Western blot were for the expression of VEGF mRNA and protein levels. ELISA was for the generation of VEGF in supernatant. Results VCR (0. 5 and 2. 0 μg/mL) or Endo (2. 0 μg/mL) used separcitely inhibited the cell proliferation and promoted the apoptosis, and also inhibited the expression and generation of VEGF. When the cells were treated with VCR and Endo together, the inhibitory effect of them were significantly strengthened. Conclusion The combination of VCR and Endostar synergistically inhibit the expression and generation of VEGF and induces the apoptosis in PLA-802 cells.关键词
Endostar/长春新碱/PLA-802细胞/血管内皮生长因子Key words
Endostar/ VCR/ PLA-802 cell/ VEGF分类
医药卫生引用本文复制引用
杨清平,周知,吕波,陶勇,李胜难,罗小红,殷清华..小剂量VCR联合Endostar抑制PLA-802细胞系VEGF表达[J].基础医学与临床,2011,31(1):32-36,5.基金项目
重庆市卫生局科研项目(2008-2-381) （2008-2-381）
