南方农业学报2011，Vol.42Issue(2)：192-196,5.
凡纳滨对虾类泛素折叠修饰蛋白Ufm1基因克隆、序列分析及结构预测
Cloning and sequencing of ubiquitin-fold modifier l(Ufml) gene and its structure prediction in Litopenaeus vannamei
摘要
Abstract
[Objective]The present experiment was conducted to analyze the structural characteristics and protein function of ubiquitin-fold modifier Ⅰ(Ufml) gene. [Method]The muscular tissue of Litopenaeus vannamei family with growth character segregation was used to construct the subtracted eDNA libraries of growth traits in Litopenaeus vannamei and obtain Ufml gene using dot blot technique. The full length of Ufml gene sequence in Liltopenaeus vannamei was screened from a full length cDNA library. [Result]The full length of cDNA sequence of Ufml gene was 714 bp, and contained a 261 bp open reading frame (ORF) encoding 86 amino acids (aa). The predicted molecular weight was 9.3 ku and the theoretical isoelectric point was 6.55. The bioinformatics analysis results revealed that the predicted encoding protein had no signal peptide and notable transmembrane region. It may be located in the cytoplasm and belonged to the hydrophitia protein. The protein contained a casein kinase Ⅱ phosphorylation loci, a N-glycosylation loci and two protein kinase C phosphorylation loci. The deduced amino acid sequence analysis results showed that Ufml genes in different species had high homology as also revealed by phylogenic tree.关键词
凡纳滨对虾/差减cDNA文库/Ufm1基因/生物信息学Key words
Litopenaeus vannamei/ subtracted cDNA libraries/ ubiquitin-fold modifier I gene/ bioinformatics分类
农业科技引用本文复制引用
盛小伟,高永华,彭金霞,李咏梅,陈晓汉,熊建华..凡纳滨对虾类泛素折叠修饰蛋白Ufm1基因克隆、序列分析及结构预测[J].南方农业学报,2011,42(2):192-196,5.基金项目
国家科技支撑计划项目(2008BADB9B03) （2008BADB9B03）
国家虾产业技术体系岗位科学家经费项目(nycytx-46) （nycytx-46）
广西直属公益性科研院所基本科研业务项目(2060302GXIF-2008-02) （2060302GXIF-2008-02）
