神经损伤与功能重建2011，Vol.6Issue(2)：79-83,5.DOI:10.3870/sjsscj.2011.02.001
重组人促红细胞生成素体外诱导人脐静脉内皮细胞血管新生作用的实验研究
Angiogenic Role of Recombinant Human Erythropoietin on EA. hy926 Cell Line: an invitro Study
姚晓艳 1李红戈1
作者信息
- 1. 华中科技大学同济医学院附属协和医院,武汉,430022
- 折叠
摘要
Abstract
Objective: To study the angiogenic role of recombinant human erythropoietin (rH-EPO) on EA. hy926 cells after oxygen and glucose deprivation followed by reoxygenation (OGD/R) in vitro. Methods: Cells were subjected to OGD/R and stimulated by rH-EPO during the reoxygenation procedure. MTT assay, Transwell assay and Matrigel assay were used to detect the angiogenic ability of cells post-OGD. The expressions of vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS) mRNA and eNOS protein were also detected. Results: OGD induced a significant decrease in proliferation, migration, and tube formation ability of EA. hy926 cells and the expression of VEGF, eNOS mRNA and protein were significantly decreased. rH-EPO mitigated the OGD-induced damage of proliferation, migration, and tube formation ability of cells and increased the expression of VEGF, eNOS mRNA and protein.Conclusion: VEGF and eNOS probably play critical roles in the angiogenic effects of rH-EPO on cells after OGD.关键词
重组人促红细胞生成素/血管新生/氧糖剥夺/血管内皮生长因子/内皮型一氧化氮合酶Key words
recombinant human erythropoietin/ angiogenesis/ oxygen glucose deprivation/ vascular endothelial growth factor/ endothelial nitric oxide synthase分类
医药卫生引用本文复制引用
姚晓艳,李红戈..重组人促红细胞生成素体外诱导人脐静脉内皮细胞血管新生作用的实验研究[J].神经损伤与功能重建,2011,6(2):79-83,5.
