南方农业学报2011，Vol.42Issue(2)：134-136,3.
罗汉果鲜花粉培养条件研究
Optimization of culture conditions for fresh pollens of Siraitia grosvenorii
摘要
Abstract
[Objective]The experiments were conducted to investigate the suitable culture conditions for the fresh pollen of Siraitia grosvenorii. [ Methods ]The combinations of different concentrations of sucrose and boric acid, pH and temperatures were used to culture the fresh pollen of Siraitia grosvenorii in different time, and the germination rate of pollen was determined. [ Results ]The best optimum medium to culture the fresh pollen of Siraitia grosvenorii was found to be the sucrose (15％)+boric acid (200 mg/L), pH 6.5. The germination rate of fresh pollen in this medium reached 77.6％on the same day, when cultured at 27～32℃. The same germination rate were recorded when cultured at 5-8℃, which decreased to 52.8％ after 15 days of culture. However, it was suitable in case of the asynchronous flowering period and dispatching pollen to different regions. [Conclusion]The germination rate of Siraitia grosvenorii pollen declined with the elongation of culture time. Relative lower temperature could make the germination rate decrease significantly slowly. Boric acid could promote Siraitia grosvenorii pollen germination and pollen tube elongation, and enhanced the pollen germination rate, but Siraitia grosvenorii pollens were unsuitable for culturing in alkaline environment.关键词
罗汉果/花粉/培养基/萌发率Key words
Siraitia grosvenorii/ pollen/ medium/ germination rate分类
农业科技引用本文复制引用
贾红林,周凤珏,许鸿源,万秀勇,何冰,梁琼月,龚银花..罗汉果鲜花粉培养条件研究[J].南方农业学报,2011,42(2):134-136,3.基金项目
广西青年基金项目(桂科青0640009) （桂科青0640009）
广西教育厅科研项目(TLZ100719) （TLZ100719）
