南方农业学报2011，Vol.42Issue(2)：205-208,4.
氯霉素残留一步式化学发光酶免疫法的建立
A one-step mircorplate chemiluminescent enzyme immunoassay for detection of residual chloramphenicol
摘要
Abstract
[Objective]The present study was aimed to establish a convenient, rapid and accurate method for detection of residual chloramphenicol (CAP). [ Method ]Based on conventional two-step chemiluminescent enzyme immunoassay (CLEIA), the two antibodies were added to solution at the same time, and the antigen coating conditions, antibody eon centration, competitive reaction timing, etc., were optimized to establish one-step competitive indirect ehemilnaineseent enzyme immunoassay. [Result ]The established one-step CLEIA was utilized to detect the CAP, and the best detection was found in range of 0.045-128.000 μg/L with 3.16 μg/L detection sensitivity, and 0.01 μg/L lowest detection limit. This method could detect the CAP residual value even below the lowest limit of international standard. As compared to the twostep CLEIA, one-step CLEIA could lessen 1.5 hour of reaction time on the premise of no effects on detection limit and sensitivity. [Conclusion ]The established one-step CLEIA method produced the same quality of detection results for residual CAP as produced by two-step CLEIA, while it has advantage of taking lesser time compared to the later one.关键词
氯霉素/一步式化学发光酶免疫法/灵敏度(IC50)/残留Key words
ehloramphenicol/ one-step CLEIA/ sensitivity (IC50)/ residue分类
轻工纺织引用本文复制引用
马玲,关忠谊,吴健敏,黄红梅,张启模,蒋维维..氯霉素残留一步式化学发光酶免疫法的建立[J].南方农业学报,2011,42(2):205-208,4.基金项目
广西创新能力建设项目(08-05-01D) （08-05-01D）
广西基本科研业务费专项项目(桂兽研专项08-4) （桂兽研专项08-4）
