神经损伤与功能重建2011，Vol.6Issue(2)：90-93,4.DOI:10.3870/sjsscj.2011.02.003
远志总皂苷对神经干细胞向神经元分化的影响
Effects of Polygalaceae Saponins on Neural Stem Cell Differentiation into Neurons
摘要
Abstract
Objective: To observe the effects of polygalaceae saponins (TEN) on neural stem cells (NSCs) differentiation into neurons. Methods: NSCs were isolated from rat hippocampus, cultured and identified. TEN with a final concentration of 5 μg/mL, 20 μg/mL, and 100 μg/mL was respectively used to induce differentiation of NSCs. The expression of neuron specific enolase (NSE) was detected by cell immunocytochemistry at days 1 and 3 after induction.Results: Primary and sub-cultured cells proliferated to form neurospheres. After treatment by TEN for 1 or 3 days, the expression of NSE was significantly increased (P＜0.01). Conclusion:TEN can enhence the differentiation of the hippocampal NSCs into neurons.关键词
神经干细胞/远志总皂苷/神经元/分化Key words
neural stem cells/ polygalaceae saponins/ neuron/ differentiation分类
医药卫生引用本文复制引用
黄昕艳,王禹,张晓梅,赵锦程,朱晓峰..远志总皂苷对神经干细胞向神经元分化的影响[J].神经损伤与功能重建,2011,6(2):90-93,4.基金项目
国家自然科学基金(No.30971039) （No.30971039）
