寄生虫与医学昆虫学报2011，Vol.18Issue(1)：48-51,4.DOI:10.3969/j.issn.1005-0507.2011.01.010
牛瑟氏泰勒虫病PCR诊断方法的建立
Establishment of a PCR Assay on Theleria sergenti in Cattle
白光彦 1杨艳玲 1张西臣 2尹继刚 2陈峙峰3
作者信息
- 1. 吉林市丰满区小白山乡畜禽防疫服务中心,吉林丰满132108
- 2. 吉林大学畜牧兽医学院,长春130062
- 3. 吉林大学人兽共患病研究所,长春130062
- 折叠
摘要
Abstract
A PCR test was developed to detect Theleria sergenti in caule which proved to be a quick and sensitive methods.A pair of synthetic oligonucleotide primers, designed from the gene encoding a 32 kDa surface protein of T. sergenti, was used to amplify parasite DNA from the blood of T. sergenti-infected cattle by the Polymerase Chain Reaction ( PCR) . The results showed that a predicted 875 bp DNA product was achieved. The detection with PCR for T. sergenti is specific because of no amplification in B. equi, bovine leukocyte and T. annulata. As shown in results of sensitivity for PCR. the minimal number of DNA copies waa 4. 5 according to the maximal template DNA dilution of 1: 100 000. Moreover, of 50 specimens from gazing cattle, 18 were microscopically positive, whereas PCR revealed 31 samples were positive.Therefore, PCR provides a useful diagnostic tool for detecting T. sergenti infected cattle, and it is significantly more sensitive than the current microscope methods.关键词
牛瑟氏泰勒虫/PCR/基因组DNA/P32/诊断方法引用本文复制引用
白光彦,杨艳玲,张西臣,尹继刚,陈峙峰..牛瑟氏泰勒虫病PCR诊断方法的建立[J].寄生虫与医学昆虫学报,2011,18(1):48-51,4.
