江西农业大学学报2011，Vol.33Issue(1)：43-46,83,5.
RT-PCR法特异性快速检测黄瓜绿斑驳花叶病毒
Rapid Detection of Cucumber green mottle mosaic virus by RT-PCR
摘要
Abstract
In this study specific primers were designed based on the sequence of movement protein gene, a rapid and effective method for cucumber green mottle mosaic virus detection was established. The established detection system amplified band with expected size of 591 bp in infected leaves, but the other 9 viruses of Tobamovirus were not. The method,which can also detect CGMMV in seeds,is rapid, accurate and sensitive, providing a reliable technical support for entry -exit inspection and quarantine and safety of agricultural production.关键词
黄瓜绿斑驳花叶病毒/运动蛋白基因/特异性检测分类
农业科技引用本文复制引用
张卫东,权永兵,廖力,王建国,徐淼锋,迟远丽..RT-PCR法特异性快速检测黄瓜绿斑驳花叶病毒[J].江西农业大学学报,2011,33(1):43-46,83,5.基金项目
国家质量监督检验检疫总局课题(2008IK264)资助 （2008IK264）
