江西农业大学学报2011，Vol.33Issue(1)：128-136,9.
番鸭呼肠孤病毒YB株σNS基因的克隆和序列分析
Cloning and Sequence Analysis of the σNS Gene of Muscovy Duck Reovirus YB Strain
摘要
Abstract
The σNS gene of muscovy duck reovirus YB( DRV- YB)was amplified by RT- PCR with oligonucleotide primers, which were designed based on previously reported σNS sequences of avian and muscovy duck reoviruses in the GenBank. The PCR product was cloned into the pMD18 -T vector and confirmed by PCR and sequencing analyses. The results show that the σNS gene of DRV -YB contains 1 191 bp,with conserved 5' and 3 ' terminal motifs typical of avian reoviruses. It contains one open reading frame from nt 24 to nt 1 127, which encodes a protein of 367 amino acids. Further nucleotide and amino acid sequence alignments among DRV - YB, DRV - 89026, and ARV - S1133 indicate that the σNS nucleotide sequence of DRV - YB is 87.3％ and 76.5％ homologous to the corresponding genes in DRV -89026 and ARV-S1133, while the deduced amino acid sequence of DRV -YB is 94.8％ and 90.5％ homologous to DRV -89026 and ARV -S1133,respectively. Phylogenetic analysis shows that the σNS genes of DRV - YB and DRV - 89026 are closely related to and fall into the same evolutionary cluster. DRV - YB and DRV - 89026 as well as DRV - 89330 share the same characteristics of S -class genes, with the same gene length and encoded proteins. However, the genome organization of DRV -YB is dramatically different from those of ARV -S1133 and ARV - 176, which are strains from chicken. The results suggest that the S - class genes exhibit polymorphism in the length of gene segments and encoded proteins among different Avian orthoreovirus strains. This polymorphism may reflect exchange and recombination of gene segments among different Avian orthoreovirus strains in nature.关键词
番鸭呼肠孤病毒/σNS基因/克隆/序列分析分类
农业科技引用本文复制引用
吴异健,王劭,黄一帆,吴宝成..番鸭呼肠孤病毒YB株σNS基因的克隆和序列分析[J].江西农业大学学报,2011,33(1):128-136,9.基金项目
福建省自然科学基金重大项目(2002F003) （2002F003）
