江西农业大学学报2011，Vol.33Issue(1)：147-150,4.
野葛块根总RNA的不同提取方法比较研究
Comparison of Different Methods for Total RNA Extraction from Pueraia Earthnut
摘要
Abstract
In this paper, the wild type and the mutant type of pueraia earthnut were used as the experimental material to compare the effects of Trizol, CTAB, SDS and modified SDS on total RNA extraction. Through analyzing the yield, purity, quality and electrophoretograms, an improved effective SDS method was preliminary developed to extract the total RNA from pueraia earthernut. The OD260/OD280 value of RNA extracted with this method varied in a range of 1. 7 ～ 1.9 and the eleetrophoretograms were integrated. This RNA extraction method is characterized by the high yield, low cost, etc. , and could be used in some experiments, such as RT - PCR, cDNA- AFLP and northern hybridization.关键词
葛块根/总RNA提取/Trizol法/CTAB法/SDS法/改良SDS法分类
农业科技引用本文复制引用
肖旭峰,姜文轩,吴才君,范淑英..野葛块根总RNA的不同提取方法比较研究[J].江西农业大学学报,2011,33(1):147-150,4.基金项目
国家自然科学基金项目(30760142) （30760142）
