实用医学杂志2011，Vol.27Issue(2)：192-194,3.DOI:10.3969/j.issn.1006-5725.2011.02.010
高糖对大鼠肾系膜细胞Smad2/3及Smad7表达的影响
Effect of high glucose on the expressions of Smad2/3 and Smad7 in glomerular mesangial cells of rats cultured in uotro
摘要
Abstract
Objective To observe the expressions of Smad2/3 and Smad7 in glomerular mesangial cells (GMC) of rats under high glucose of different concentrations, and to investigate the change of Smad signal pathway under diabetic mellitus in rats. Methods GMC of rats cultured in votro were divided into control group (the concentration of glucose was 5.6 mmol/L), high glucose group (20 mmol/L and 30 mmol/L respectively), and mannitol group. The expressions of Smad2/3 and Smad7 in each group were measured by indirect immunofluorescence and laser scanning confocal microscope. Results The expression of Smad2/3 in control group was weak, whereas that of Smad7 was strong. The expressions of Smad2/3 in high glucose group were stronger than that in control group (P ＜ 0.05 ), while those of Smad7 in high glucose group were weaker than that in control group (P ＜ 0.05 ), both were in concentration-dependent manner. There were not significant differences of the expressions of Smad2/3 and Smad7 between mannitol group and control group. Conclusion High glucose can up-regulate the expression of Smad2/3 and down-regulate Smad7 in GMC of rats, indicating Smad signal pathway is involved in the pathogenesis of diabetic nephropathy.关键词
糖尿病/高糖/肾小球系膜细胞/Smad2/3/Smad7引用本文复制引用
马红艳,肖斌,杨军,顾峻菱,万沁,徐勇..高糖对大鼠肾系膜细胞Smad2/3及Smad7表达的影响[J].实用医学杂志,2011,27(2):192-194,3.基金项目
国家自然科学基金资助项目(编号:30670980) （编号:30670980）
