生物加工过程2011,Vol.9Issue(1):29-34,6.DOI:10.3969/j.issn.1672-3678.2011.01.007
L-谷氨酰胺高产菌的选育和发酵条件
Breeding of L-glutamine high producing strain and its fermentation conditions
摘要
Abstract
The original strain LG-3 was mutated by ultraviolet radiation and diethyl sulfate, and screened in culture dish having high concentration of sulfaguanidine or ( NH4 )2SO4.A strain LG-65 was obtained for producing 43.5 g/L of L-glutamine.The fermentation conditions were further optimized, the yield reached 47 -48 g/L in shaker flask for 72 h and reached 55 g/L cultivated in 7 L fermentor for 40 h.关键词
育种/发酵/L-谷氨酰胺/诱变分类
生物科学引用本文复制引用
柴进凯,张伟国..L-谷氨酰胺高产菌的选育和发酵条件[J].生物加工过程,2011,9(1):29-34,6.基金项目
国家高技术研究发展计划(863计划)资助项目(2006AA0203014-10) (863计划)