生物加工过程2011,Vol.9Issue(1):1-4,4.DOI:10.3969/j.issn.1672-3678.2011.01.001
中和剂对稀酸蒸爆玉米秸秆酶解效果的影响
Effects of neutralizing agents on enzymatic hydrolysis of corn stover pretreated by dilute acid-mediated steam explosion
摘要
Abstract
Com stovers pretreated by dilute sulphuric acid-mediated steam explosion were neutralized to pH 5 with Ca(OH) 2, NaOH and NH40H, or alternatively washed with water to the same pH. The cellulose conversion rates by enzymatic hydrolysis under the conditions of solide liquid ratio of 1: 10 and cellulases loading of 14 U/g cellulose were investigated. The results showed that after enzymatic hydrolysis for 72 h, the cellulose conversion rates of the cooresponding samples were up to 91.7%, 80. 7%, 83. 1%and 81.7%, respectively. Meanwhile, the effects of various factors on the efficency of cellulase hysrolysis were also discussed. Considering the cost and following fermentation process, the neutralizing agent NH4OH was the choice for the industrialization production.关键词
玉米秸秆/酶水解/中和/蒸爆分类
生物科学引用本文复制引用
张红漫,赵晶,林增祥,黄和..中和剂对稀酸蒸爆玉米秸秆酶解效果的影响[J].生物加工过程,2011,9(1):1-4,4.基金项目
国家自然科学基金资助项目(20876078) (20876078)
国家高技术研究发展计划(863计划)资助项目(2009AA02Z08) (863计划)
NSFC-广东联合基金项目(U0733001) (U0733001)
中石化项目(207035) (207035)