生物加工过程2011，Vol.9Issue(1)：19-23,5.DOI:10.3969/j.issn.1672-3678.2011.01.005
苦瓜多糖的提取、分离纯化及组成性质
Extraction, isolation, purification and composition of Momordica charantia polysaccharide
摘要
Abstract
Crude polysaccharides were extracted from Momordica charantia with hot water. Three main fractions of Momordica charantia polysaccharides(MCP): MCP1 ,MCP2 and MCP3 were obtained through purification on DEAE-32 ion exchange column. The fractions were further isolated by Sephacryl S-400 gel-permeation chromatography. The results by Sephacryl S-400 and HPLC showed that MCP1 and MCP2 were both homogeneous. The molecular weight of MCP1 and MCP2 were 1.16 × 106 and 7.45 × l05, respectively measured by high performance gel permeation chromatography. The components of MCPs were determined by HPLC after PMP-derivatization. The results showed that MCP1 was heteropolysaccharides composed of Man, Rham, GlcUA, GalUA, Glu, Gal, Xyl and Ara, with respective molar ratios of 1.03 :2.93: 1.00: 14. 95:2. 16: 30. 70: 2. 85: 4. 50. MCP2 was composed of Rham, GalUA, Gal, Xyl and Ara, with respective molar ratios of 1.63: 21.88: 4. 66: 1.00: 1.29. No typical absorption of nucleic acid or protein was detected by UV scanning.关键词
苦瓜多糖/分离纯化/单糖/衍生化分类
生物科学引用本文复制引用
吴勋贵,王泽根,蔡寅,杨烨,王旻..苦瓜多糖的提取、分离纯化及组成性质[J].生物加工过程,2011,9(1):19-23,5.基金项目
江苏省六大人才高峰资助项目 （）
