兰州大学学报（医学版）2011，Vol.37Issue(1)：39-43,5.
听神经脱髓鞘病变中的听力学和病理改变
Experimental study of hearing injury of auditory nerve demyelination in allergic demyelinating model
摘要
Abstract
Objective To establish a model of allergic demyelination, observing changes in audiology and inner ear pathology, and to explore the pathological changes in auditory neuropathy.Methods 100 Wistar rats were randomly divided into model group, the control group and normal group in order to establish the animal model of autoimmune demyelination by heterologous myelin basic protein of peripheral nerve and complete freunds adjuvant. In the control group, antigen was replaced by the complete freunds adjuvant and there was 0.9％ saline in the normal group.Auditory brainstem response audiometery, otoacoustic emission were assayed weekly before and after immunization for a period of seven weeks. Every week two rats from each group were killed randomly to observed pathological changes in auditory nerve and cochler. Results The auditory nerve in model group suffered from demyelinating lesions in the first week after delivery and the suffering reached the peak after three weeks. It could be self relieved and get a basic recovery in 6 weeks; there were no significant changes in the synaptic cleft between the inner hair cells and auditory nerve fibers. The auditory brainstem response audiometery threshold response increased from the range of 10 to 25 dBnHI in 37/120 ears in the model group, and latency of wave?prolonged in hearing loss group. For those rats, the amplitude of distortion product otoacoustic emissions reduced at 0.5, 1.0, 2.0 kHz of the hearing loss animals, and transient evoked otoacoustic emissions had same changes at all frequencies. Conclusion The immune demyelinating model has part of auditory nerve demyelination and injury of inner hair cells and its audiological changes are different from those of auditory neuropathy.关键词
听神经病/脱髓鞘疾病/诱发电位Key words
auditory neuropathy/ demyelinating diseases/ evoked potential分类
医药卫生引用本文复制引用
仇志强,陈小婉,张淑慧,张小兵,王有虎..听神经脱髓鞘病变中的听力学和病理改变[J].兰州大学学报（医学版）,2011,37(1):39-43,5.基金项目
甘肃省自然科学基金(0710RJZA035) （0710RJZA035）
