山东医药2011，Vol.51Issue(2)：22-23,2.
塞来昔布对宫颈癌HeLa细胞的放射增敏作用观察及机制探讨
Effect and mechanism of celecoxib on radiosensitivity enhancement in cervical cancer HeLa cells
摘要
Abstract
Objective To observe the effect of celecoxib on radiosensitivity enhancement in cervical cancer HeLa cells and explore its mechanism. Methods Logarithmic phase HeLa cells were divided into 3 groups, the negative control group were culture medium, radiotherapy alone group was treated with 6 MV X ray radiation therapy, radiation plus drug group was treated with 20,40 μmol / L of celecoxib, for 72 h before radiation therapy. The average lethal dose ( Do), Quasi-field dose (Dq), 2 Gy irradiation of the surviving fraction (SF2) and radiosensitization ratio (SER) was measured by colony formation assay cells radiosensitivity parameters, the expression of cyclooxygenase-2、VEGF-C、antigen were detected by immunohistochemistry. Results Do 、 Dq and SF2 in radiation plus drug group were less than the control group (P all ＜0.05), its SERDo increased from 2.01 ±0.06 to 2.41 ± 0.08. The expreasion of COX-2, VEGF-C was significantly decreased in radiotherapy plus drug group HeLa cells ( P all ＜ 0. 05 ), compared with the negative control group and radiotherapy alone. Conclusion Celecoxib can increase the radiosensitivity of HeLa cells in cancer of the cervix ,which might be rented to the influence of celecoxib down-regulated the COX-2 、VEGF-C expression of HeLa cell.关键词
环氧合酶2抑制剂/塞来昔布/宫颈肿瘤/放射疗法/血管内皮生长因子-CKey words
cyclcoxygenase-2 inhibitor/ celecoxib/ cervical cancer/ radiation therapy/ vascular endothelial growth factor -C分类
医药卫生引用本文复制引用
王爱红,余娟娟,戚世芳,田晓予..塞来昔布对宫颈癌HeLa细胞的放射增敏作用观察及机制探讨[J].山东医药,2011,51(2):22-23,2.基金项目
河南省洛阳市科技攻关资助项目(0801059A). （0801059A）
