畜牧与兽医2011，Vol.43Issue(2)：1-3,3.
牛瑟氏泰勒虫不同靶基因PCR检测方法的比较
Comparison of different PCR methods for the target genes of bovine Theileria sergenti
摘要
Abstract
To develop a more specific and sensitive PCR method for bovine Theileria sergenti. p23 . p33, HSP70 and 18S rRNA genes of T. sergenti were selected as the target genes, while the sensitivity and specificity of the methods and detection rates of clinical samples were compared. The results showed that four kinds of primers for the target genes of bovine T. sergenti had high specificity. When the concentration of T. sergenti genomic DNA was 127 ng/μL, the minimum detectahle amount of p23 , p33 , HSF70 and 18S rRNA genes were 1×105 , 1×105 , 1×104 and 1 ×106 copies/μL, respectively. The positive rates of clinical samples were 30. 19％ ( 16/53) , 39. 62％ ( 21/53) ,47. 17％ ( 25/53) and 54. 72％ ( 29/53) , respectively. It suggested that the 18S rRNA gene as a target gene for PCR method was more specific and sensitive than the other three Senes.关键词
牛瑟氏泰勒虫/p23/p33/HSP70/18S rRNA/PCR分类
农业科技引用本文复制引用
金超,贾立军,王妍,王娜,莫添添,张守发..牛瑟氏泰勒虫不同靶基因PCR检测方法的比较[J].畜牧与兽医,2011,43(2):1-3,3.基金项目
国家自然科学基金项目(30960278). （30960278）
