兰州大学学报（医学版）2011，Vol.37Issue(1)：15-20,6.
高海拔地区大鼠严重烫伤延迟复苏后肝脏低氧诱导因子-1α和p53的表达与肝脏损伤的关系
Influence of hypoxia inducible factor-1α and p53 expression on liver injury after severe scald with delayed fluid resuscitation in rats in high altitude
摘要
Abstract
Objective To explore the influence of hypoxia-inducible factor-1α (HIF-1α) and p53 expression on liver injury after severe scald with delayed resuscitation in high altitude in rats. Methods 240 Wistar rats were employed as the experimental models (TBSA 30％, Ⅲ degree), and they were randomly divided into 3 groups in different altitudes (1 517 m and 3848 m): delayed fluid resuscitation (DFR) group, immediate fluid resuscitation (IFR) group and sham scald (SS) group. The livers of rats were harvested at 1, 6, 12, 24, 72 hours and 168 hours after severe scald respectively. The levels of HIF-1α, p53 and the apoptosis percentage in liver were detected by pathological technique, tissue microarray technique, terminal deoxynucleotidy transferasemediated deoxy-uridine triphosphate nick end labeling, immunohistochemistry and image analysis technique. Results The expression levels of HIF-1α and p53 were much higher in DFR group and IFR group than those in SS group, in 3 848 m altitude and in 1 517 m altitude, and higher in DFR group than those in IFR group (P ＜0.05). The apoptosis percentage in liver was higher in DFR group than in IFR group, and higher in 3848 m altitude than in 1 517 m altitude (P ＜0.05). Conclusion HIF-1α expression and activation of p53 apoptotic pathways may play an important role in the hypoxic injury of the liver.关键词
高海拔/大鼠/烫伤/延迟复苏/肝脏/低氧诱导因子-1α/p53蛋白Key words
high altitude/ rat/ scald/ delayed fluid resuscitation/ liver/ hypoxia inducible factor1α/ p53分类
医药卫生引用本文复制引用
刘毅,张诚,刘长海..高海拔地区大鼠严重烫伤延迟复苏后肝脏低氧诱导因子-1α和p53的表达与肝脏损伤的关系[J].兰州大学学报（医学版）,2011,37(1):15-20,6.基金项目
全军医学科技研究"十一五"计划课题(06G030) （06G030）
