山东医药2011，Vol.51Issue(3)：3-5,3.
慢性乙型肝炎病毒感染者外周血T细胞受体β链V区谱型差异研究
Study on difference of T cell receptor BV dominant usage in patients infected chronic hepatitis B
摘要
Abstract
Objective To investigate the roles of difference of T cell receptor (TCR) β chain variable region gene (BV) GDR3 sequences in the inflammation occurrence of patients infected HBV. Methods PBMCs were isolated from anticogulated blood of 9 immune tolerant chronic asymptomatic HBV carrier(AsC) (AsC group)and 12 chronic hepatitis B (CHB) patients( CHB group), and RT-PCR was proformed to amplificate 24 TCR BV families transcripts, and PCR products were genescaned by polyacrylamide gel electrophoresis, and the spectrum patterns were analyzed. Results Both groups showed T cell clonal expansion with various degree, and three perturbation patterns of T cell expansion were showed in their BV families, including monoclonicity, oligoclonicity and skewed peak patterns. The number of monoclonicity and oligoclonicitytotal perturbation families in CHB group was more than that in AsC group ( P ＜ 0.05). Conclusion CHB patients may have higher degree of T cell expansion than AsC, and this maybe involved in immunopathogensis mechanism of hepatitis B virus.关键词
肝炎病毒,慢性,乙型/T淋巴细胞/受体/免疫指纹技术Key words
hepatitis virus,chronic,type B/ T cell/ receptor/immunoscope分类
医药卫生引用本文复制引用
李咏茵,马世武,张光文,侯金林..慢性乙型肝炎病毒感染者外周血T细胞受体β链V区谱型差异研究[J].山东医药,2011,51(3):3-5,3.基金项目
国家自然科学基金资助项目(30730082,30901271) （30730082,30901271）
南方医院院长基金项目(2009C009). （2009C009）
