山东医药2011，Vol.51Issue(11)：1-3,3.
Mst1基因对人喉癌Hep2细胞增殖和凋亡的影响
Effects of Mst1 gene on the proliferation and apoptosis of laryngeal carcinoma Hep2 cells
摘要
Abstract
Objective To investigate the influence and mechanism of Mst1 gene on the proliferation and apoptosis of laryngeal carcinoma Hep2 cells. Methods The Hep2 cells were regarded as control group, and the Hep2 cells transfected with pcDNA3. 1-Mst1 as Mst1 transfected group, the Mst1 transfected group cells treated with SB203580 as SB203580 treated group. In the three groups, the inhibitory rate of cell growth was detected by MTI, the apoptosis was detected by flow cytometry, and the protein expression of Mst1 and phosphorylated p38 ( p-p38 ) and total p38 ( t-p38 ) were detected by Western blot. Results Compared with control group, the protein expression of Mst1 and p-p38 in Mst1 transfected group up-regulated significantly, t-p38 protein showed no changes, the apoptesis radio increased from 2.46％ to 7.37％, the inhibitory rate of cell growth was 41.8％. Compared with Mst1 transfected group, the protein expression of p-p38 in SB203580 treated group down-regulated significantly, t-p38 protein showed no changes, the apoptesis radio decreased from 7.37％ to 2.65％, the inhibitory rate of cell growth was - 10. 1％, cell proliferation decreased significantly. Conclusion Mst1 gene can regulate the apoptesis and proliferation of laryngeal carcinoma cells through p38 MAPK pathway.关键词
Mst1基因/喉肿瘤,喉癌/细胞凋亡分类
医药卫生引用本文复制引用
尚超,李巍,郭艳,富伟能,孙开来..Mst1基因对人喉癌Hep2细胞增殖和凋亡的影响[J].山东医药,2011,51(11):1-3,3.基金项目
国家自然科学基金资助项目(30700980). （30700980）
