山东医药2011，Vol.51Issue(12)：12-14,后插1,4.
脐带华通胶间充质干细胞对急性再生障碍性贫血T淋巴细胞亚群的影响
Effects of umbilical cord Wharton's jelly mesenchymal stem cells on T lymphocyte subsets in cases with acute aplastic anemia
沈建良 1黄友章 1宫立众 1岑坚 1赵德峰 1刘毅 1尹文杰 1郑培浩 1刘晓鹏1
作者信息
- 1. 中国人民解放军海军总医院,北京100048
- 折叠
摘要
Abstract
Objective To investigate the modulatory effects of umbilical cord Wharton' s jelly mesenchymal stem cells (UCWJMSCs) on T lymphocyte (TLC) subsets in cases with acute aplastic anemia (AAA) in vitro. Methods Peripheral blood samples collected from 21 cases with AAA and 10 healthy volunteers were processed for mononuclear cells (MNCs) suspension. UCWJMSCs and MNCs were mixed at a certain ratio and cocultured for 0-120 h. TLC subsets were detected by FCM post-coculture and analyzed statistically. Results AAA was a disease with Th1 and Tc1 polarized status. There were no changes in TLC subsets in healthy volunteers post-coculture. But TLC subsets in AAA cases changed markedly except for Th2 and Tc2, i.e. the percentages of CD3+, CD8+, CD28+, Th1 and Tc1 subsets decreased markedly, and that of CD4+,CD4+/CD8+, CD56+, CD25+ and CD103+ subsets rised significantly (P ＜0. 05). The modulatory effects produced 24 h and peaked 72 h post-coculture. The modulation depended upon the UCWJMSCs concentration. Conclusion UCWJMSCs could significantly modulate TLC subsets in AAA cases, but UCWJMSCs derived from different individuals played the same role in the modulation.关键词
脐带华通胶间充质干细胞/贫血,再生障碍性/T淋巴细胞亚群分类
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沈建良,黄友章,宫立众,岑坚,赵德峰,刘毅,尹文杰,郑培浩,刘晓鹏..脐带华通胶间充质干细胞对急性再生障碍性贫血T淋巴细胞亚群的影响[J].山东医药,2011,51(12):12-14,后插1,4.
