中国水稻科学2011，Vol.25Issue(1)：11-18,8.DOI:10.3969/j.issn.1001-7216.2011.01.002
hrcQ基因决定水稻条斑病菌在非寄主烟草上的过敏性反应和在寄主水稻上的致病性
hrcQ Gene Determines Xanthomonas oryzae pv. oryzicola to Trigger Hypersensitive Response in Nonhost Tobacco and Pathogenicity in Host Rice
摘要
Abstract
hrcQ gene of Xanthomonas oryzae pv. oryzicola (Xoc) is highly conserved in the animal and plant pathogens. It has been postulated that the differentiation at the N-terminal of HrcQ proteins in different plant pathogenic bacteria is related to the secretion of specific effectors through type Ⅲ secretion system (T3SS). However, it is unclear whether HrcQ affects the formation of T3SS and the secretion of T3SS effectors. To understand the effect of HrcQ on the formation of T3SS and the secretion of T3SS effectors, hrcO mutant of Xoc was generated by knocking-out mutagenesis through allelic exchange. Pathogenicity assays verified that the hrcO mutant lost the ability to trigger hypersensitive response (HR) in nonhost tobacco and pathogenicity in host rice. The expression of hrcQ was induced when the pathogen interacted with rice cells. The protein-protein interaction assay by yeast two-hybrid system showed that HrcQ could interact with Hpal, HrcN, HrpB5 and HrpB2.Immunobloting assay confirmed that HrcQ was not secreted through T3SS, whereas the mutation of hrcQ led to no secretion of Hpal and HrpB2 through T3SS. These results suggest that HrcQ is the core component of T3SS helping Hpal and HrpB2 secret through T3SS to determine HR in tobacco and pathogenicity in rice. All the above also provides a basis for further understanding of the secretion mechanisms by X. oryzae pv. oryzicola T3SS.关键词
水稻条斑病菌/hrcQ基因/Ⅲ型分泌系统/过敏性反应/致病性分类
生物学引用本文复制引用
张鼎鼎,邹丽芳,赵梅勤,邹华松,陈功友..hrcQ基因决定水稻条斑病菌在非寄主烟草上的过敏性反应和在寄主水稻上的致病性 [J].中国水稻科学,2011,25(1):11-18,8.基金项目
国家973计划资助项目(2006CB101902) （2006CB101902）
国家自然科学基金资助项目(30710103902) （30710103902）
国家公益性行业(农业)科研专项资助项目(NYHYZX07-056). （农业）
